DESNATURALIZACION DE LA CATALASA PDF

Atentamente Christian A. Descubrir que lo extraordinario, Lo monstruosamente anormal Es esta breve cosa que llamamos… vida Jaime Sabines, Otros poemas sueltos. Gerardo, Dra Saila y a mi amiga Heidi. A la Dra.

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Atentamente Christian A. Descubrir que lo extraordinario, Lo monstruosamente anormal Es esta breve cosa que llamamos… vida Jaime Sabines, Otros poemas sueltos. Gerardo, Dra Saila y a mi amiga Heidi. A la Dra. Isabelle Gaime, por tanta hospitalidad, a la Dra. Anne Marie Farnet y al Dr. Al equipo de Tierra Blanca, especialmente a la Dra. A Heidi. Encontramos que la lacasa en P. Encontramos que el pretratamiento con P. Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus.

Light-induced inhibition of laccase in Pycnoporus sanguineus. Laccase induction by synthetic dyes in Pycnoporus sanguineus and their use for sugar cane bagasse delignification. Applied Microbiology and Biotechnology Aceptado. Cellulase and xylanase production from different Ascomycota in mixed cultures of liquid media.

Enzyme and Microbial Technology Sometido el 26 de mayo de Laccase is inhibited by white light in Pycnoporus sanguineus. Production of fungal cellulases and xylanases in mixed cultures. Estructura de las fibras de celulosa 5.

Cultivos liquidos de P. Toma de muestras de mantillo en campo Combinaciones de cepas evaluadas para producir celulasas en cultivos liquidos. Esto se debe a que el etanol posee un octanaje mayor que la gasolina i. Figura 2. Tomado de IEA Figura 3. Figura 4. Estructura de las fibras de celulosa. Figura 5. Figura 7. Figura 8. El efecto deslignificador de los agentes alcalinos se atribuye a su capacidad para saponificar los enlaces ester contenidos entre la celulosa y la hemicelulosa con la lignina Figura 8 Sun y Cheng, Tabla 1.

Figura 9. Entre estas enzimas se encuentran la lacasa, la Mn-peroxidasa y la ligninasa Sindhu et al. Generalmente, las lacasas se inducen cuando fuentes complejas de estos nutrientes se encuentran disponibles e.

Figura Tabla 2. No determinada. Clostridium ha sido propuesto como un organismo. Esto debido a que son organismos resistentes y con gran plasticidad para crecer en diferentes sustratos y adaptarse a diversas condiciones ambientales. Generalmente, las enzimas producidas por una sola especie de hongo, poseen ciertas carencias en alguno de los requerimientos mencionados.

Este tipo de cultivos suelen llevarse a cabo en biorreactores, en condiciones controladas. Aspergillus niger puede variar su metabolismo dependiendo del tipo de cultivo que se utilice Maghsoodi y Yaghmaei, Todo esto ocurre gracias a la presencia de receptores en la membrana celular de las hifas Figura Tomado de Aro et al. Tabla 4. L-1 25 FPU. El proceso puede ser llevado a cabo tanto por bacterias e.

Zymomonas mobilis como por levaduras e. Saccharomyces cerevisae. Aunque algunos autores sugieren que es preferible fermentar inicialmente la glucosa con S. Esta enzima habilita a S. Tabla 5. L-1 de 30 4. L 30 6 Chen et al. Los productos obtenidos a partir de estos compuestos van desde solventes e. Objetivos 5. Producto de etapa: Etanol celulosico. La etapa 1 y 2 se realizaron de manera independiente y convergen en la etapa 3.

Los cultivos liquidos inoculados con un cuadro 0. Camaras de luz LED construidas para evaluar el efecto de exponer a diferentes longitudes de onda de luz al hongo P. La actividad lacasa fue determinada de acuerdo a Criquet Anexo L-1 o ausencia de etanol 0 g. Micelio de P. Los cultivos utilizados como pretratamiento se realizaron en bolsas de polipapel. Sin embargo, la mayor limitante de este proceso es que las enzimas comercialmente disponibles, producidas con el hongo T. L-1 para inhibir el crecimiento de bacterias.

Trichoderma sp. Las morfoespecies detectadas fueron aisladas secuencialmente hasta obtener cultivos puros. Los muestreos se realizaron cada 24 horas durante 96 horas Pereira et al. Light-induced inhibition of laccase in Pycnoporus sanguineus 1 Christian A. Abstract The aim was to determine which specific regions of light stimuli [measured as wavelengths and lx] and that it does the visible light spectrum were responsible for the induction or so inversely. This can be explained as an ecological mecha- inhibition of laccase in Pycnoporus sanguineus.

Cultures were nism of environmental recognition, given that P. The presence of short wavelength light — nm nm , and darkness. The results indicate that short wave- would indicate that the organism finds itself in an external lengths strongly inhibit the production of laccase: green 3.

This possible new regulation L in proportions of It is concluded that the production of laccase in P. Culturas Veracruzanas , Col.

Emiliano Zapata, Xalapa, Veracruz , Mexico of the resources at hand. Pycnoporus sanguineus 2 Polyporaceae is a basidiomycete fungus that, as a saprobe, Faculty of Biology, Universidad Veracruzana, Cto.

Emiliano Zapata, Xalapa, Veracruz , Mexico produces extracellular enzymes that allow it to decompose 3 organic polymers and consume its monomers. Laccases are enzymes with a wide specificity of substratum involved in. Some of A single-factor design was used to determine the effect of their applications are the pretreatment of wood for biofuel exposition to different types of visible light on the production production and the recycling or transformation of toxic waste of laccase in P.

The mushroom was exposed to and other pollutants such as polycyclic aromatic hydrocar- different light wavelengths within the visible light spectrum: bons, pesticides, and industrial dyes Kunamneni et al. It is and nm , and red nm , using light-emitting diodes known that the production of laccases is affected by chemical LEDs. As a means of control, cultivars kept in conditions of signals; for example, a high abundance of simple carbonated darkness were used.

This is attributed to the carbon catabolic Fisher Scientific , corresponding to Such transcription regulators have the glucose consumption kinetics and the final biomass pro- been characterized in the laccase gene LAC1 - promoting duction were also quantified.

Samples for the determination of region of basidiomycete fungi Janusz et al. At the end of the experiment, the significant effects of the However, in spite of the fact that light has been associ- ated treatments were determined by the one-way variance analysis with regulation in the production of exoenzymes and with protocol [ANOVA; Zar ] and the Fisher LSD Least various bioprocesses in fungi such as sporulation and significant difference post hoc test. Also, in order to know if regulation of circadian rhythms Saha et al.

This study sets out to sion analysis were done. We therefore set out to find evi- Cultivar medium and conditions dence of enzymatic regulation, measured as inhibition, resulting from wavelength changes within the visible light The experimental units consisted of glass containers with spectrum.

Strain and inoculum Analytic methods The strain of P. The strain was preserved added. As inoculum, during the experi- syringaldazine per min U per one L of cultivar U. Subsequently, the mixture was diluted with 4 mL of distilled Treatment Wavelength Biomass Glucose Laccase H2O and quantified at nm in a spectrophotometer.

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